Sterilization is a process that eliminates or kills all forms of microbiology includind fungi, bacteria, viruses and spore. Sterilization can be used by different method such as heat, irradiation,chemical,pressure or filtration.
1.Steam sterilization basic principle:
Steam sterilization has been used for more than a century to destroy microbiology. Steam sterilization destroy microbiology by misture and high temperature. The basic principle of steam sterilization is to expose item to direct steam contact at the high temperature and pressure for the specified time.
2.Autoclave
Autoclaves are widely used in microbiology , medicine , podiatry , tattooing , body piercing , veterinary science , mycology , dentistry , and prosthetics fabrication. Autoclave is a sterilizes tool through a process of heated and pressurized steam. Means to create absolute condition of total destruction of elimination of all living microorganisms.
Fig 1 Autoclave is the process design to produce a sterile state.
3.How to use autoclave4.Uses of steam sterilization
Steam sterilization can be used on all ctitical and semi-critical items that are heat and moisture stable.
More information:
1.Guideline for Disinfection and Sterilization in Healthcare Facilities, 2008
2.WHO - Infection Control Guidelines for Transmissible Spongiform Encephalopathies
Writte by Chang,yi-chu (Blythe)

